Journal: Human Vaccines & Immunotherapeutics

Article Title: Neutralizing activity of anti-respiratory syncytial virus monoclonal antibody produced in Nicotiana benthamiana

doi: 10.1080/21645515.2024.2327142

Figure Lengend Snippet: Schematic representation of the T-DNA region of the pBYR2e RSV-F Fc fusion plant expression vector. The T-DNA region plays a crucial role in facilitating the transfer of the gene of interest into plant cells. It includes the left border (LB) and right border (RB) which serve as the boundaries for gene transfer. The Pin II 3’ sequence derived from potato proteinase inhibitor II acts as a border element helping to facilitate the insertion of the desired genes into the plant genome. The vector also incorporates several important components, such as the Tomato Bushy Stunt Virus (TBSV) RNA silencing suppressor, P19; the Cauliflower Mosaic Virus (CaMV) 35s promoter, P35s; the CaMV enhancer, P35s×2; the tobacco extension gene region, Ext3’ FL, 3‘; the tobacco RB7 promoter, Rb7 5’ del; the Bean Yellow Dwarf Virus (BeYDV) short intergenic region, SIR; the BeYDV long intergenic region, LIR; and the BeYDV replication initiation proteins, Rep and RepA, along with C2/C1. .

Article Snippet: Human RSV fusion glycoprotein was detected with rabbit anti-RSV-F mAb (Cat:11049-R302-H, Sino Biological, Beijing, China) diluted 1:3000 in PBS containing 0.5% BSA and 0.01% Tween-20 added to each well and incubated for 1 h at 37°C.

Techniques: Expressing, Plasmid Preparation, Sequencing, Derivative Assay, Virus

Journal: Human Vaccines & Immunotherapeutics

Article Title: Neutralizing activity of anti-respiratory syncytial virus monoclonal antibody produced in Nicotiana benthamiana

doi: 10.1080/21645515.2024.2327142

Figure Lengend Snippet: Binding characteristics of plant-produced anti-RSV mAbs. ELISA was used to quantify the specific binding to the RSV-F his-tagged monomer protein. The purified plant produced anti-RSV mAbs, and the plant produced nivolumab (anti PD-1, as a negative control). The binding activity of the antibody was detected with HRP-conjugated anti-human IgG antibody. The data are shown as the mean of triplicates and error bars represent standard deviation. The EC 50 was calculated by using GraphPad Prism software 9.3.

Article Snippet: Human RSV fusion glycoprotein was detected with rabbit anti-RSV-F mAb (Cat:11049-R302-H, Sino Biological, Beijing, China) diluted 1:3000 in PBS containing 0.5% BSA and 0.01% Tween-20 added to each well and incubated for 1 h at 37°C.

Techniques: Binding Assay, Produced, Enzyme-linked Immunosorbent Assay, Purification, Negative Control, Activity Assay, Standard Deviation, Software